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Good sample preparation prior to 2-D electrophoresis is critical for producing meaningful, reproducible results. Extracting and solubilizing proteins, eliminating contaminants, and reducing sample complexity ensure proper separation and analysis.
Bio-Rad has applied its expertise in solution chemistry, chromatography, and electrophoresis to develop the most comprehensive suite of products available for protein sample extraction, cleanup, fractionation, and depletion.
Success depends on sample purity. Contaminants such as salts, detergents, and ionic compounds can ruin a 2-D experiment, so it is crucial to eliminate these contaminants prior to analysis. Bio-Rad provides a variety of effective extraction and cleanup options to ensure high-quality results.
Fractionation and depletion reduce sample complexity and enrich samples for proteins of interest. This can greatly improve visualization of low-abundance proteins by removing other proteins that mask their detection. Bio-Rad offers a wide range of approaches for fractionating complex protein mixtures and depleting high-abundance proteins.
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