Salt removal using the ReadyPrep 2-D cleanup kit. E. coli extracts containing 1 M NaCl were electrophoresed before and after treatment with the 2-D cleanup kit. The samples were focused using 11 cm ReadyStrip pH 3–10 IPG strips, then run on Criterion 8–16% Tris-HCl precast gels for the second dimension.

Removal of salts reduces streaking and improves reproducibility of 2-D gels.

ReadyPrep 2-D Cleanup Kit

• Uses TCA-like precipitation for salt removal
• Can concentrate protein samples
• Available in two sizes — for 5 and 50 preps

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Bio-Spin and Micro Bio-Spin 6 Columns

• Provide fast salt removal in an easy-to-use spin-column format
• Remove compounds <6 kD by size exclusion chromatography
• Accommodate up to 100 µl of sample

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