Detergent removal using the ReadyPrep 2-D cleanup kit. E. coli extracts containing 1% SDS were electrophoresed before and after treatment with the 2-D cleanup kit. The samples were focused using 11 cm ReadyStrip pH 3–10 IPG strips, then run on Criterion 8–16% Tris-HCl precast gels for the second dimension.

Ionic contaminants such as detergents, lipids, and phenolic compounds interfere with 2-D resolution and reproducibility.

ReadyPrep 2-D Cleanup Kit

• Uses TCA-like precipitation to wash away contaminants
• Can concentrate protein samples
• Available in two sizes — for 5 and 50 preps

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