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Differences in 2-D patterns obtained using ReadyPrep
signal (A), membrane I (B), and membrane II (C) kits. Mouse liver samples
were extracted using the recommended protocols for each kit. Purified protein
(~450 µg) was focused on 17 cm pH 3–10 nonlinear ReadyStrip
IPG strips and run on 8–16% PROTEAN II precast gels for the second dimension.
Overall spot patterns differ for A, B, and C even though all three kits isolate
membrane proteins, indicating each kit isolates different types of membrane
proteins.
Bio-Rad offers a variety of choices for fractionation by cellular location.
- Enriches cytoplasmic and nuclear proteins
- Isolates nuclei, then extracts with a strongly chaotropic buffer
- Uses a quick and effective protocol
- Does not require ultracentrifugation or preparation of density gradients
- Isolates more complex membrane proteins than the membrane I kit
- Isolates membrane proteins involved in membrane
trafficking and signaling
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