Distribution of proteins based on differential solubility using the ReadyPrep sequential extraction kit. The generation of three fractions provides increased resolution of proteins on 2-D gels.

Bio-Rad provides two options to reduce sample complexity using differential solubilization.

ReadyPrep Sequential Extraction Kit

• Divides protein samples into 3 fractions by differential solubilization
• Uses the detergent SB 3-10 in a strong solubilizing solution

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ReadyPrep Protein Extraction Kit (Soluble/Insoluble)

• Divides protein samples into 2 fractions
• Uses the detergent ASB-14 (a stronger detergent than SB 3-10) in a strong solubilizing solution

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