Fractionation of rat brain tissue using
Aurum ion exchange mini columns. Rat brain total protein extracts
(3 ml) were loaded onto an Aurum AEX (anion exchange) or CEX (cation exchange) mini column and eluted. The
unfractionated and fractionated samples
were then treated with the ReadyPrep
reduction-alkylation and 2-D cleanup kits
and separated by 2-D gel electrophoresis.
Fractionation by charge allows separation of acidic and basic proteins.
- Fractionate acidic and basic proteins, respectively, by ion exchange chromatography
- Use a patented polymerization technology to achieve ultrahigh protein binding capacity
- Accommodate up to 1 ml of sample
- Use a quick, easy spin-column format
