Clean fractionation by pI. Mouse liver extract was fractionated using
the MicroRotofor cell. 2-D separations of
the unfractionated sample (120 µg protein)
and fraction 3 (20 µg protein) are shown.
Prior to 2-D separation, samples were
treated with the ReadyPrep 2-D cleanup kit.
Note the clean pH boundaries of fraction
3 and the enrichment of proteins in the pH
region it covers.
Protein fractionation by pI improves downstream sample loading and separation on
narrow- and micro-range IPG strips by eliminating proteins outside the pH region of interest.
The Rotofor and mini Rotofor cells fractionate proteins by preparative liquid-phase IEF and:
- Separate and concentrate proteins into 20 discrete fractions
- Separate up to 1 g of protein
- Complement your studies and downstream protein purification strategies
- Offer temperature regulation to improve reproducibility and preserve protein structure and function
- Can be used with custom pH gradients
Based on Rotofor cell technology, the compact MicroRotofor system performs liquid-phase IEF with the following unique key features:
- 2.5 ml sample capacity
- 10 liquid fractions
- Easy setup
- Temperature regulation
