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The following are links to widely used protein extraction protocols. As more researchers focus on
proteomics, extraction protocols for a variety of new tissues, plants, bacteria, and other
microorganisms will be developed. These links are a start.
Animal Tissue
Protein
extraction (Bottone protocol, University of North Carolina, Chapel Hill)
Protein
isolation using Trizol reagent (Bottone protocol, University of North Carolina, Chapel Hill)
Plants
Protein preparation
from plant tissue (Orlic protocol, Michigan State University)
Protein
preparation from rice (Brutnell Lab, Boyce Thompson Institute, Cornell University)
Bacteria and Other Microorganisms
Protein
extraction from Aspergillus nidulans (Osherov and May protocol, Baylor
College of Medicine)
Simple
method for preparation of E. coli protein for SDS-PAGE (Hahn Lab, Fred Hutchinson Cancer
Research Center and Howard Hughes Medical Institute)
Preparation
of active proteins from inclusion bodies (Vuillard and Freeman protocol, Northwest Fisheries
Science Center web site)
2-D Electrophoresis for Proteomics: A Methods and Product Manual
Download PDF
Modification of Bio-Rad DC Protein Assay for Use With Thiols
Download PDF
Total Cellular Protein Determination Using the New DC Protein Assay
Download PDF
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