Enrichment of low-abundance serum proteins with the ProteoMiner protein enrichment kit. Resolution is dramatically improved on a gel with an equal amount of total protein from sample treated with ProteoMiner beads. The high-abundance proteins are greatly reduced and a greater number of protein spots are visualized. Samples (100 µg protein) were focused on 11 cm ReadyStrip pH 5–8 IPG strips, then run on Criterion 8–16% Tris-HCI precast gels for the second dimension.
Removing high-abundance proteins leads to enrichment of proteins of lower abundance. ProteoMiner technology is based on the interaction of proteins with a large, highly diverse library of hexapeptides bound to chromatographic supports. It can be used with virtually any sample type.
- Enrich medium- and low-abundance proteins
- Decrease the amount of high-abundance proteins without immunodepletion
- Are not dependent on a predefined set of antibodies, unlike immunodepletion products
- Minimize the potential co-depletion of proteins bound to high-abundance proteins
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