Albumin and IgG removal from serum using the Aurum serum protein mini kit. Serum proteins were electrophoresed before and after treatment with an Aurum serum protein mini column. Albumin and lgG are removed following treatment with an Aurum serum protein column, improving resolution of other protein spots. Samples (100 µg protein) were focused on 11 cm ReadyStrip pH 5–8 IPG strips, then run on Criterion 8–16% Tris-HCl precast gels for the second dimension.

Proteomic analysis of serum presents its own unique challenges. Albumin and IgG contribute up to 90% of the total protein in a serum sample. These proteins obscure lower-abundance proteins and limit loading capacity on 2-D gels. To obtain meaningful results, these proteins must be removed prior to downstream separation or analysis.

Aurum Affi-Gel Blue and Aurum Serum Protein Mini Kits and Columns

• Easily and effectively remove albumin, or both albumin and IgG, by affinity chromatography
• Increase sample load of other proteins 3- to 4-fold
• Use a quick and easy spin-column format
• Provide eluted proteins ready for IEF analysis

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