Protocols

The following are links to widely used protein extraction protocols. As more researchers focus on proteomics, extraction protocols for a variety of new tissues, plants, bacteria, and other microorganisms will be developed. These links are a start.

Animal Tissue

Protein extraction (Bottone protocol, University of North Carolina, Chapel Hill)

Protein isolation using Trizol reagent (Bottone protocol, University of North Carolina, Chapel Hill)

Plants

Protein preparation from plant tissue (Orlic protocol, Michigan State University)

Protein preparation from rice (Brutnell Lab, Boyce Thompson Institute, Cornell University)

Bacteria and Other Microorganisms

Protein extraction from Aspergillus nidulans (Osherov and May protocol, Baylor College of Medicine)

Simple method for preparation of E. coli protein for SDS-PAGE (Hahn Lab, Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute)

Preparation of active proteins from inclusion bodies (Vuillard and Freeman protocol, Northwest Fisheries Science Center web site)

2-D Electrophoresis for Proteomics: A Methods and Product Manual
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Modification of Bio-Rad DC Protein Assay for Use With Thiols
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Total Cellular Protein Determination Using the New DC Protein Assay
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